Influence of molecular weight on NCI CPTC Antibody Characterization Program. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. ; De Jong, S.J. (1969). In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. Dilute Solution. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. ; Garca de , J. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. ; Rani, K.Y. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Colloid and Polymer Science, 286, 1223-1231. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). Webwhittier union high school district superintendent. Granath, K. A. Intrinsic viscosity of bovine serum albumin conformers. dextran hydrodynamic radius. a-d Haematoxylin/eosin-stained histological sections of human cadaver skin sample and e quantified data using ImageJ for different time periods after microporation. Figure5 shows the cumulative amount of the FITC-dextran (M.W. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). The receptor chamber was filled with PBS. Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Treatment of acne scars by fractional bipolar radiofrequency energy. (2013). Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. It is well tolerated on parenteral infusion by most people but a very low incidence This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. and transmitted securely. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Schematic illustration of transdermal delivery after the application of the microporation device. In order to confirm the molecular weight the gel permeation chromatography (GPC) for standard dextrans is used in a range of molecular weights from 8.8 to 2000kDa and these correspond to the following equation. Fractional thermolysis by bipolar radiofrequency facilitates cutaneous delivery of peptide and siRNA with minor loss of barrier function. Molecular dynamics simulations on aqueous ACS Nano. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Amersham Biosciences, AA Edition, www.amershambiosciences.com. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. 4K, 10K, and 20K) permeated through the human, and pig skin after microporation. The qM-H values given by equation 13, these vary from 0.9993 to 0.9841 for the range between 8.8 to 200kDa in a temperature range of 20 to 40C. dextran The flattening of the curves at higher molecular weight is in agreement with an increased branching density. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Small Intestinal Permeability and Gut-Transit Time Determined On single point determination of intrinsic viscosity. European Polymer Journal, 42, 1086-1092. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Journal of Applied Polymer Science, 13, 2031-2032. AATEX. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Article 2011;5(2):95132. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. 10K, and c M.W. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. Chem. WebIn this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. International Journal of Biological Macromolecules, 42, 133-137. Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of. This result could reflect the presence of some aggregated species within the lysozyme sample solution. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. Synthesis and characterization of blue dextrans. Provided by the Springer Nature SharedIt content-sharing initiative. Radiofrequency with microneedling. The values of 0 decreases from 2.5429 to 2.3326 x 1023mol-1 demonstrating a relative flexibility of particles. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Herwadkar A, Banga AK. Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor. Webof overall macromolecular geometry and hydrodynamic draining. The effect of branching is best recognized from the molecular weight dependencies of []. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. The human skins were from the back or thigh of a 24-year-old woman. where c is the solute concentration (g/cm3), [] is the intrinsic viscosity (cm3/g), and kH represents Huggins constants (cm3/g). (1991). To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. On the molecular weight distribution of dextran T-500. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Similar results are obtained in references [35, 36, 37], using a system of dextran / ethylene glycol, and dextran/dimethylsulfoxide, respectively. Physicochemical studies of oligodextran. WebTry out PMC Labs and tell us what you think. Hindered diffusion of dextran and ficoll in microporous membranes. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. In the future, we plan to investigate the possibility of enhancing the delivery of other macromolecular drugs such as peptides and siRNAs using the RF-based microporation device. principles of dynamic light scattering What is dextran? Application areas - TdB Labs AB Correspondence to A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. ; Talsma, H.; Hennink, W.E. MeSH terms The calculation of Mark-Houwink parameters is carried out by the graphic representation of the following equation: where kMH (cm3/g) and a are Mark-Houwink constants, depending on the type of polymer, solvent, and temperature of intrinsic viscosity determinations [46]. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Carbohydrate Polymers, 78, 902-907. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Structural and biocompatibility properties of dextran from National Library of Medicine J. Braz. (2006). Tirtaatmadja, V.; Dunstan, D. E.; Boger, D. V. (2001). dextran hydrodynamic radius Careers. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. of different origins. 2009;6(4):34354. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. 2015;32(5):170413. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Polymolecularity and polydispersity in Molecular weight determinations. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish volume24, Articlenumber:22 (2020) Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. We observe that this maximum radius is below (2000). The enhanced permeability through micropores was confirmed by Franz diffusion cell. Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Critical parameters for gel permeation 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Dilute solution properties and degree of chain branching Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Journal of Applied Polymer Science, 72, 871-876. Ann Surg Oncol. Garcia de la Torre, J.; Carrasco, B. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. 2013;3(5):3588605. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. (2003). Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Sachdeva V, Banga AK. Carbohydrate Polymers 88 1440-1444. 2012;9(2):14754. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. The Intrinsic Viscosity of Biological Macromolecules. 2019;26(1):25261. Learn More. Dextran Skin barrier disruption by acetone: observations in a hairless mouse skin model. Ind. By using this website, you agree to our Barrier integrity was examined using Tewameter to confirm the poration of human cadaver skin using a microporation device. Molecular weight determination of polysaccharides. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows International Journal of Pharmaceutics, 277, 99-104. DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). SWC designed and coordinated the research. Dilute solution properties and degree of chain branching for dextran. Gillespie, T.; Hulme, M. A. dextran This conjecture found to be correct. (1999). International Journal of Biological Macromolecules, 23, 135-141. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. 2009;8(2):1739. (2004). Walkenhorst, S.; Olivier, R. (2002). Drug Deliv. The .gov means its official. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Google Scholar. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Polymers | Free Full-Text | Confinement-Induced Fractionation and Weissella: An Emerging Bacterium with Promising Health Benefits. 8580 Macromolecules 34, Notes - NIST The skin poration was performed using the microporation device (LG electronics, Korea). Epub 2012 May 28. J Appl Toxicol. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Hydrodynamic Properties of Tragacanthin. Intrinsic viscosity molecular weight relationship. Carbohydrate Polymers, 79, 380-390. Crini, G. (2005). Citation-(RIS Search Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Chem. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. WebR H Hydrodynamic radius [m] The basic DLS setup. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. He, L.; Niemeyer, B. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Drug Deliv. Cell permeabilization using a non-thermal plasma - IOPscience Ficoll and dextran vs. globular proteins as probes for testing (2013). Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Polysaccharides-based nanoparticles as drug delivery systems. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. (2008). The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Privacy A solvent at this temperature is called a theta solvent [53]. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Transdermal delivery of FITC-Dextrans with different molecular Table 2 shows that comparing the intrinsic viscosity data obtained from the Huggins method and the Solomon-Ciuta single-point method, a similar values are obtained and the %RE is lower for low Mw and it increases for higher Mw. Hydrodynamic Carbohydrate Polymers 88 373-381. WebThe invention relates to novel fluorescent dyes with multiple negatively charged groups in their ionized form which are 9-aminoacridines or 1-aminopyrene shaving of one of the fol Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter (d h ~ 70 nm) on order of the collagen mesh size. Hydrodynamic radii were measured using Dynamic Light Scattering (DLS) in a Malvern Zetasizer NanoZS DLS (Malvern Instruments, Malvern, UK) at high scattering angle (173) to minimise the effects of dust and supramolecular contamination (NIBS, 173). Journal of Physical Chemistry, 58(11), 953-957. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. ; Dutt, N.V.K. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. These results suggest that fibroblasts proliferate and migrate under the SC, and regenerate the skin. Normally, the skin loses very little water loss whereas damaged skin loses more [22]. 2021 Aug;13(4):915-925. doi: 10.1007/s12602-021-09751-1. However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of PubMedGoogle Scholar. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. If plotted for all molecular weights, the double logarithm of [] and Mw, the value of a obtained in all cases shows a value smaller than 0.5, which reports that dextran macromolecule has a degree of hyperbranched with increasing molecular weight and increases much for higher Mw (< 200kDa) which making the application of M-H method more difficult. 1905/05) for their financial support. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Chem. Polysaccharide hydrogels for modified release formulations. At 20C data "a" value obtained in this work is intermediate to those given by references [1, 37] while the value of kM-H is lower. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. dextran
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